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. 2014 Dec 31;7:493. doi: 10.1186/s13068-014-0183-x

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© Hasunuma et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Molecular characterization of the parental (GT), flv3 -overexpressing (Flv3ox), and vector control (VC) strains. (a) Genome structure around integration site in glucose-tolerant (GT), Flv3ox, and vector control (VC) strains. Pr1 and Pr2 indicate the positions of the primer used for the PCR screening. Cm ^r, chloramphenicol resistance cassette; PpsbA2, psbA2 promoter. (b) Genomic PCR analysis of GT, Flv3, and VC strains using Pr1 and Pr2. (c) Immunoblot analysis of Flv3 protein in GT, Flv3ox, and VC cells. Total soluble protein extracted from 1.6 mg DCW Synechocystis cells was reacted with anti-Flv3 antibody after separation on SDS-polyacrylamide gel. Phycocyanin was detected by staining with Coomassie brilliant blue. The amount of protein loaded on the gel is 75 μg (GT), 63 μg (Flv3ox), and 70 μg (VC).