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. 2015 Jan 13;11:1. doi: 10.1186/s12917-014-0312-6

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© Wang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Evaluation of the sensitivities of nanoPCR (a) and conventional PCR (b) for the detection of MEV NS1 plasmid DNA. Lane M: Low DNA Mass Ladder (Invitrogen, Carlsbad, USA); lanes 1–9: different MEV NS1 plasmid DNA copies subjected to nanoPCR and conventional PCR (8.75 × 10⁸, 8.75 × 10⁷, 8.75 × 10⁶, 8.75 × 10⁵, 8.75 × 10⁴, 8.75 × 10³, 8.75 × 10², 8.75 × 10¹, and 8.75 × 10⁰ copies/μL, respectively); lane 10: blank.