Table 2. Primers used in this study.

Primers used to generate the mutants
Name	Sequence	Primer pair	Introduced restriction sites	Destination
MsGR1rnhA	TCGAGGGCAAGCTGCGCGAC	MsGR2rnhA	-	Gene replacement rnhA
MsGR2rnhA	CGTAGCACCGCACCCCAGCC	MsGR1rnhA	-	Gene replacement rnhA
MsGR3rnhA	CGGGATCCGTGCGCGCGCCACCAGGTC	MsGR4rnhA	BamHI	Gene replacement rnhA
MsGR4rnhA	GAAGCTTCCGCGAGGGGCCGAACACC	MsGR3rnhA	HindIII	Gene replacement rnhA
GR1MsRnhAII-KpnI	GGTACCCCGCCGACGATGATGCTGTC	GR2MsRnhAII-BamH	KpnI	Gene replacement MSMEG4305
GR2MsRnhAII-BamH	CAAGCGGCGCAACGGGATC	GR1MsRnhAII-KpnI	-	Gene replacement MSMEG4305
GR3MsRnhAII-BamH	CGGGATCCTACACAACCGCGCCGTAGCC	GR4MsRnhAII-Hind	BamHI	Gene replacement MSMEG4305
GR4MsRnhAII-Hind	GCAAGCTTTCGCTGCTGGGTGCCGTGAC	GR3MsRnhAII-BamH	HindIII	Gene replacement MSMEG4305
MsGR1rnhB	AACTGCAGACTACCTGCGCGAGCTCCGTG	MsGR2rnhB	PstI	Gene replacement rnhB
MsGR2rnhB	GAAGCTTCGCAGACCCGACGATTTCCG	MsGR1rnhB	HindIII	Gene replacement rnhB
RnHBgr3a	GCGAATTCCGTCGCTTCCCGTGATCGGC	RnHBgr4a	HindIII	Gene replacement rnhB
RnHBgr4a	GGGGTACCAAGGGTTTTGCCGCATCCGC	RnHBgr3a	KpnI	Gene replacement rnhB
MsA1–5562-BglIIs	CAGATCTGTGAACCACCGGCACCACGCC	MsA1–5562-XbaI	BglII	Complementation rnhA
MsA1–5562-XbaI	CTCTAGATGGTGGTCGGCCTGGCGGG	MsA1–5562-BglIIs	XbaI	Complementation rnhA
MsrnhAIIPace-sBglII	CAGATCTGTGAAGGTTCTCGTCGAGGCCGAC	MsrnhAII-rev-Xba	BglII	Complementation MSMEG4305
MsrnhAII-rev-Xba	CTCTAGATGCACTCGTGAGCTACAGGTACGC	MsrnhAIIPace-sBglII	XbaI	Complementation MSMEG4305