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. 2015 Jan 20;10(1):e0114954. doi: 10.1371/journal.pone.0114954

Figure 8. Cerberus suppresses breast cancer cell colony-forming ability.

Figure 8

(A) Representative images of colony formation assay for MCF-7 (left) and MDA-MB-231 cells (right) (10X magnification). Cells were grown in serum containing medium supplemented with 0 nM (top, control), 17.8 nM (not shown) or 178 nM (bottom) Cerberus. (B) Analysis of colony formation assay for MCF-7 (right) and MDA-MB-231 (left) cells (3A). Images were analyzed using ImageJ to determine number of colonies. Experiments were carried out with 0 nM (blue), 17.8 nM (red), or 178 nM (green) Cerberus. Colony formation assays were performed in triplicates in 6 well plates.