Table 1. Primers Used for Real-Time Polymerase Chain Reaction.
Gene | Sequence (5’–3’) | Melting Temperature (°C) |
---|---|---|
18S | F GTAACCCGTTGAACCCCATT | 60 |
R CCATCCAATCGGTAGTAGCG | ||
Osteopontin | F TTCCAAGTAAGTCCAACGAAAG | 60 |
R GTGACCAGTTCATCAGATTCAT | ||
BSP | F ATGGCCTGTGCTTTCTCAATG | 60 |
R GGATAAAAGTAGGCATGCTTG | ||
Runx2 | F ATGCTTCATTCGCCTCAC | 60 |
R ACTGCTTGCAGCCTTAAAT | ||
Osteocalcin | F ATGAGAGCCCTCACACTCCTCG | 60 |
R GTCAGCCAACTCGTCACAGTCC | ||
ALP | F CGTGGCTAAGAATGTCATCATGTT | 60 |
R AGGGGAACTTGTCCATCTCC | ||
BMP2 | F AGTTGCGGCTGCTCAGCATGTT | 60 |
R CCGGGTTGTTTTCCCACT | ||
BMPR1a | F TTTATGGCACCCAAGGAAAG | 60 |
R TGGTATTCAAGGGCACATCA | ||
BMPR1b | F AAAGGTCGCTATGGGGAAGT | 60 |
R GCAGCAATGAAACCCAAAAT | ||
BMPR2 | F CATCCGAACCCTCTCTTGAT | 60 |
R TGCATAAAGATCCATTGGGA |
BSP, bone sialoprotein; Runx2, runt-related transcription factor 2; ALP, alkaline phosphatase; BMP2, bone morphogenetic protein 2; BMPR, BMP receptor