Table 1. Primers Used for Real-Time Polymerase Chain Reaction.

Gene	Sequence (5’–3’)	Melting Temperature (°C)
18S	F GTAACCCGTTGAACCCCATT	60
	R CCATCCAATCGGTAGTAGCG
Osteopontin	F TTCCAAGTAAGTCCAACGAAAG	60
	R GTGACCAGTTCATCAGATTCAT
BSP	F ATGGCCTGTGCTTTCTCAATG	60
	R GGATAAAAGTAGGCATGCTTG
Runx2	F ATGCTTCATTCGCCTCAC	60
	R ACTGCTTGCAGCCTTAAAT
Osteocalcin	F ATGAGAGCCCTCACACTCCTCG	60
	R GTCAGCCAACTCGTCACAGTCC
ALP	F CGTGGCTAAGAATGTCATCATGTT	60
	R AGGGGAACTTGTCCATCTCC
BMP2	F AGTTGCGGCTGCTCAGCATGTT	60
	R CCGGGTTGTTTTCCCACT
BMPR1a	F TTTATGGCACCCAAGGAAAG	60
	R TGGTATTCAAGGGCACATCA
BMPR1b	F AAAGGTCGCTATGGGGAAGT	60
	R GCAGCAATGAAACCCAAAAT
BMPR2	F CATCCGAACCCTCTCTTGAT	60
	R TGCATAAAGATCCATTGGGA

BSP, bone sialoprotein; Runx2, runt-related transcription factor 2; ALP, alkaline phosphatase; BMP2, bone morphogenetic protein 2; BMPR, BMP receptor