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. 2015 Jan 20;10(1):e0116895. doi: 10.1371/journal.pone.0116895

Figure 1. Expression of PTEN and PIK3R3, and degree of Akt phosphorylation, in Ewing Sarcoma cell lines.

Figure 1

The indicated Ewing Sarcoma cell lines (TC71, SK-N-MC, SK-ES-1, EWS502 and A673) were cultured in their respective media, and harvested at similar confluence (~70%) for protein extract preparation. Expression levels of PIK3R3, PTEN, pAkt (Ser473) and (total) Akt were determined using immunoblotting, with tubulin as the loading control; PIK3R3/tubulin ratios were determined by densitometry, with the ratio in TC71 cells arbitrarily set to 1.