Combining MeDIP-seq and MRE-seq to investigate genome-wide CpG methylation

. Author manuscript; available in PMC: 2016 Jan 15.

Published in final edited form as: Methods. 2014 Nov 6;72:29–40. doi: 10.1016/j.ymeth.2014.10.032

Buffer	Composition
Extraction buffer	50 mM Tris-HCl pH 8.0
	1 mM EDTA pH 8.0
	0.5% SDS
	1 mg/ml proteinase K (add fresh)
0.1 M Na₂HPO₄/NaH₂PO₄ (pH 7.0)	61 mM Na₂HPO₄
	39 mM NaH₂PO₄,
10% Triton X-100	10 ml Triton X-100
	fill up with 90 ml ultrapure water
MeDIP wash buffer	10 mM Na₂HPO₄/NaH₂PO₄ (pH7.0)
(prepare fresh)	140 mM NaCl
	0.05% Triton X-100
MeDIP elution buffer	0.25 mg/ml proteinase K
(prepare fresh)	0.25% SDS
	fill up with TE buffer
0.1% Tween-20	100 µl Tween-20
	fill up with 99.9 ml ultrapure water