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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Aquat Toxicol. 2014 Dec 18;159:184–197. doi: 10.1016/j.aquatox.2014.12.012

Figure 7. Relationship between mRNAs of ERαS (A, C, E) or ERαL (B, D, F) and estrogen responsive mRNAs (Vtg: A, B; AroB: C, D; total ERα: E, F) in individual killifish from SC (all) as compared to NBH fish expressing ERαΔ6 and/or ERαΔ6–8 ERα deletion variants (NBH+del) or all other remaining NBH fish (NBH-del).

Figure 7

Adult male and female killifish from NBH or SC were injected with estradiol or vehicle alone and tissues processed for qPCR analysis (see legend to Figure 4). Results were expressed as MNE and log transformed. Analysis of covariance (ANCOVA) was applied separately by site and the presence of deletion variants (SC, NBH+del and NBH-del). For each estrogen responsive mRNA (Y-axis, dependent variable), ERαS or ERαL mRNA was plotted on the X-axis (independent variable). See Table 4 for statistical analysis of the relationships, and Table 5 for significance between regression lines for different population pairs.