Figure 7.
Measurement of mitochondrial Ca2+ uptake in CTRL and Mfn2 KD FDB fibers after incubation with either 20µM EGTA-AM or 20µM BAPTA-AM. A. Average (±SE) time course of mitochondrial rhod-2 fluorescence in FDB fibers at rest, during 5 successive tetanic stimuli, and for 10 minutes of recovery following the 5th and final tetanus in CTRL (black circles) or Mfn2 KD fibers (grey circles). B. Average (±SE) relative myoplasmic mag-fluo-4 fluorescence in CTRL or Mfn2 KD fibers during a single 500ms tetanus.