Figure 2.

Metabolic syndrome (MetS) is associated with increased osteoclastogenesis and bone resorption induced by lipopolysaccharide (LPS). C57BL/6 mice, with or without MetS, were injected with phosphate-buffered saline (PBS) or LPS in periodontal tissue to induce periodontitis (as described in Methods). After the maxillae were examined by micro–computed tomography (as shown in Figure 1), they were decalcified and sectioned. Tartrate-resistance acid phosphatase (TRAP) staining to detect osteoclasts was performed on the tissue sections. Representative tissue sections from 4 groups were shown (A), and multinucleated TRAP-positive osteoclasts were quantified (B). The data are presented as mean ± SD (n = 7). B, alveolar bone; T, tooth.