Figure 3.

Metabolic syndrome (MetS) is associated with increased leukocyte infiltration and bone resorption induced by lipopolysaccharide (LPS). In addition to tartrate-resistance acid phosphatase staining, the tissue sections were stained with hematoxylin and eosin. Furthermore, histologic evaluation was performed of leukocyte infiltration and bone resorption in subepithelial gingival (A) and periodontal ligament (B) tissue after treatment with phosphate-buffered saline (PBS) or LPS in mice, with or without MetS. Photomicrographs were taken at 200× magnification. The inflammatory scores for leukocyte infiltration and bone resorption were determined (C) according to the criteria described in Methods. The data are presented as means ± SD (n = 7). B, alveolar bone; T, tooth.