Figure 7.

Functional evaluation of Mash1 transactivation properties upon overexpression of p57kip2. A, Determination of the percentage of CNPase-positive oligodendroglial cells upon combinatorial overexpression of an empty vector as control (pIRES), overexpression of Mash1 (pIRES_Mash1), and overexpression of p57kip2 (pIRES_p57) after 3 d in culture. B–D′′, Representative immunofluorescent CNPase staining of cultured cells upon combinatorial gene overexpression. E, Quantitative real-time RT-PCR illustrating the relative expression levels of the Mash1 reporter plasmid tdTomato upon combinatorial transfection of expression vectors pIRES (empty control), pIRES_Mash1, and pIRES_p57kip2. Data are means derived from n = 3 experiments ± SEM (total number of counted cells: 1177 in A; t test: *p < 0.05, **p < 0.01). Scale bars, 30 μm.