Figure 9.

Colocalization of binding proteins upon overexpression of wild-type or NLS mutant p57kip2 proteins. Examination of cytoplasmic localization of LIMK-1 (A–C′′), CDK2 (D–F′′), and transcription factor Hes5 (M–O′′), and percentage of cells positive for Sox10 (J–L′′) and Mash1 (G–I′′). LIMK-1, CDK2, and Hes5 follow subcellular translocation of p57kip2, whereas Sox10 and Mash1 remained in the nucleus, the latter of which was downregulated and upregulated upon wild-type and NLS mutant p57kip2 overexpression, respectively. Data are means derived from n = 4 experiments ± SEM (total number of counted cells: 677 in A; 569 in D; 541 in G; 546 in J; 415 in M; t test: *p < 0.05, **p < 0.01, ***p < 0.001). Scale bars, 20 μm.