Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 21;35(3):972–984. doi: 10.1523/JNEUROSCI.2641-14.2015

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Genheden et al.

This article is freely available online through the J Neurosci Author Open Choice option.

PMC Copyright notice

Figure 4. — Inhibition of MNKs by CGP57380 or MNK-I1 blocks the BDNF-induced increases in phospho-eIF4E and global protein synthesis. A, Representative Western blots for the indicated proteins or phosphoproteins. B, C, The effect of treating neurons with CGP57380 before BDNF stimulation on eIF4E and ERK phoshporylation. two-way (BDNF × CGP57380) ANOVAs revealed main effects of BDNF on phospho-eIF4E (F_(1,28) = 4.9, p = 0.03) and phospho-ERK (F_(1,20) = 205, p < 0.001), a main effect of CGP57380 on phospho-eIF4E (F_(1,28) = 142, p < 0.001) but not phospho-ERK (p = 0.78), and an interaction between BDNF and CGP57380 administration on phospho-eIF4E (F_(1,28) = 9.6, p = 0.004) but not phospho-ERK (p = 0.94); n = 6–8. D, Neurons were treated with CGP57380 before BDNF stimulation in the presence of [³⁵S]methionine. A two-way (BDNF × CGP57380) ANOVA revealed a main effect of BDNF (F_(1,45) = 4.1, p = 0.05), a main effect of CGP57380 (F_(1,45) = 18.9, p < 0.001), and an interaction between BDNF and CGP57380 administration (F_(1,45) = 4.7, p = 0.04); n = 11–12. E, Representative Western blots for parts (F) and (G). F, G, The effect of treating neurons with MNK-I1 before BDNF stimulation on eIF4E and ERK phosphorylation. Two-way (BDNF × MNK-I1) ANOVAs revealed main effects of BDNF on phospho-eIF4E (F_(1,16) = 12.7, p = 0.003) and phospho-ERK (F_(1,24) = 194, p < 0.001), a main effect of MNK-I1 on phospho-eIF4E (F_(1,16) = 55.8, p < 0.001) but not phospho-ERK (p = 0.36), and an interaction between BDNF and MNK-I1 administration on phospho-eIF4E (F_(1,16) = 9.8, p = 0.006) but not phospho-ERK (p = 0.37); n = 5. H, Neurons were treated with MNK-I1 before BDNF stimulation in the presence of [³⁵S]methionine. A two-way (BDNF × MNK-I1) ANOVA revealed a main effect of BDNF (F_(1,56) = 8.8, p = 0.005), a main effect of MNK-I1 (F_(1,56) = 42.0, p < 0.001), and an interaction between BDNF and MNK-I1 administration (F_(1,56) = 5.0, p = 0.028); n = 12; *p < 0.05 compared with NT. Error bars represent SEM.