Figure 7.

Summary of proteins identified as sensitive to MNK1 inhibition. A, All proteins identified in two of three replicates and their corresponding heavy medium (H/M) (MNK1 WT/KO) ratio. Dashed lines indicate thresholds for 1.46, 1.78, and 2.18 enrichment of samples in MNK1 WTs over KOs, which correspond to cutoffs of 1, 1.5, and 2 SD over the mean of all H/M ratios. B, Histogram summary of SILAC data. C, Venn diagram illustrating the overlap of the 718 proteins identified in the present study, the 842 high confidence FMRP targets from (Darnell et al., 2011), and the MNK1-sensitive proteins above the 1.5 SD threshold. D, Proteins increased (red) or decreased (blue) by at least 1.5 SD in response to BDNF in WT versus MNK1 KO neurons. Proteins that have also been identified as high-confidence FMRP targets are represented by diamonds. Interactions were determined using the STRING database (v9.1) with a high confidence (0.7) threshold (Franceschini et al., 2013).