Table 1. IC₅₀ concentrations of loperamide in canine cancer cells.

	CTAC	D-17	CML-1	CMT-12
IC50 (µM) 24 hr	20 ± 0.8	8.7 ± 0.5	19.4 ± 0.6	27 ± 0.5
IC50 (µM) 48 hr	16.2 ± 0.8	8.2 ± 0.8	16.7 ± 1.1	25.5 ± 1.7
IC50 (µM) 72 hr	19.3 ± 0.5	7.2 ± 0.3	14.8 ± 1.1	25.8 ± 0.3

IC₅₀ concentrations for 4 canine cancer cell lines after 24, 48 and 72 hr of incubation with loperamide. There was no difference in IC₅₀ based on time, as analyzed via ANOVA with Tukey post-hoc test. Values are expressed as mean ± SEM from three independent experiments.