Table 2. Sample numbers and the distribution of the mite samples which showed positive in PCR for detection of avian pathogen DNAs from the mite samples without washing.
| Prefectures | Sample number |
Single pathogen positive | Multiple pathogens positive | All negative (%) |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| APVa) | MSb) | MGc) | Subtotal (%) | APV Wild & MS Vac (%) |
APV Wild & MS Wild (%) |
APV Vac & MS Vac (%) |
APV Wild & MG Vac (%) |
Subtotal (%) |
||||||
| Wild (%) |
Vacd) (%) |
Wild (%) |
Vac (%) |
Wild %) |
Vac (%) |
|||||||||
| Akita | 1 | 1 (100) | 0 | 0 | 0 | 0 | 0 | 1 (100) | 0 | 0 | 0 | 0 | 0 | 0 |
| Aomori | 2 | 0 | 0 | 1 (50.0) | 0 | 0 | 0 | 1 (50.0) | 0 | 0 | 0 | 0 | 0 | 1 (50.0) |
| Chiba | 44 | 5 (11.4) | 0 | 1 (2.3) | 0 | 0 | 1 (2.3) | 7 (15.9) | 1 (2.3) | 0 | 0 | 0 | 1 (2.3) | 36 (81.8) |
| Fukui | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 (50.0) | 0 | 0 | 1 (50.0) | 1 (50.0) |
| Gunma | 6 | 1 (16.7) | 0 | 0 | 0 | 0 | 0 | 1 (16.7) | 0 | 0 | 0 | 0 | 0 | 5 (83.3) |
| Hokkaido | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 (100) | 0 | 0 | 1 (100) | 0 |
| Hyogo | 11 | 2 (18.2) | 0 | 1 (9.1) | 0 | 0 | 0 | 3 (27.3) | 0 | 1 (9.1) | 0 | 0 | 1 (9.1) | 7 (63.6) |
| Ibaraki | 6 | 1 (16.7) | 0 | 0 | 0 | 0 | 0 | 1 (16.7) | 1 (16.7) | 0 | 0 | 0 | 1 (16.7) | 4 (66.7) |
| Iwate | 7 | 0 | 0 | 1 (14.3) | 0 | 0 | 0 | 1 (14.3) | 0 | 0 | 0 | 0 | 0 | 6 (85.7) |
| Kanagawa | 3 | 1 (33.3) | 0 | 1 (33.3) | 1 (33.3) | 0 | 0 | 3 (100) | 0 | 0 | 0 | 0 | 0 | 0 |
| Mie | 12 | 1 (8.3) | 0 | 0 | 0 | 0 | 0 | 1 (8.3) | 0 | 0 | 1 (8.3) | 1 (8.3) | 2 (16.7) | 9 (75.0) |
| Nagasaki | 6 | 0 | 1 (16.7) | 0 | 1 (16.7) | 0 | 0 | 2 (33.3) | 0 | 0 | 0 | 0 | 0 | 4 (66.7) |
| Okayama | 8 | 0 | 1 (12.5) | 0 | 1 (12.5) | 0 | 0 | 2 (25.0) | 0 | 0 | 0 | 0 | 0 | 6 (75.0) |
| Shizuoka | 3 | 1 (33.3) | 0 | 0 | 0 | 0 | 0 | 1 (33.3) | 0 | 0 | 0 | 0 | 0 | 2 (66.7) |
| Tochigi | 3 | 0 | 0 | 0 | 1 (33.3) | 0 | 0 | 1 (33.3) | 0 | 0 | 0 | 0 | 0 | 2 (66.7) |
| Other 23 prefecturese) | 44 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 44 (100) |
| Total | 159 | 13 (8.2) | 2 (1.3) | 5 (3.1) | 4 (2.5) | 0 | 1 (0.6) | 25 (15.7) | 2 (1.3) | 3 (1.9) | 1 (0.6) | 1 (0.6) | 7 (4.4) | 127 (79.9) |
Erysipelothrix rhusiopathiae, Salmonella enterica, Fowl Adenovirus and Marek’s disease virus were not detected. a) If the intact RE provirus integration was detected, such samples were considered as wild type. If the intact RE provirus integration was not detected, such samples were considered as vaccine. b) If 16S rRNA detected was different from the sequence of vaccine used in Japan, such samples were considered as wild type. If 16S rRNA detected was identical to the sequence of vaccine used in Japan, such samples were considered as vaccine. c) If mgc2 sequence detected was different from the sequence of vaccine used in Japan, such samples were considered as wild type. If mgc2 sequence detected was identical to the sequence of vaccine used in Japan, such samples were considered as vaccine. d) Vac: vaccine. e) Other 23 prefectures: Aichi, Ehime, Fukushima, Gifu, Hiroshima, Ishikawa, Kagoshima, Kumamoto, Kyoto, Miyagi, Miyazaki, Nara, Niigata, Oita, Saga, Saitama, Tokushima, Tottori, Toyama, Wakayama, Yamagata, Yamaguchi and Yamanashi.