Fig. 1.

Effects of various concentrations of estradiol (black bars) or STX (gray bars) in DMEM containing 1 nM GnRH on luteinizing hormone (LH) secretion from cultured bovine AP cells. The LH concentrations in control cells (cultured medium only) were averaged with the mean value set at 100%. The LH concentrations of the treated groups are expressed as a percentage of the control. Each value represents the mean concentration ± SEM (n=12). ⁺P<0.05, ⁺⁺P<0.01: significant differences compared with the control, ^†P<0.05, ^††P<0.01: significant difference compared with GnRH, **P<0.01: significant differences compared with 10 nM estradiol.