Skip to main content
. Author manuscript; available in PMC: 2015 Jan 21.
Published in final edited form as: Nat Struct Mol Biol. 2014 Mar 23;21(4):366–374. doi: 10.1038/nsmb.2796

Figure 5. Transcriptional activation of an unexpressed gene leads to repair by HR.

Figure 5

a. PolII-S2P profile obtained in DIvA cells is shown on the GBP cluster of gene, located on chromosome 1. A TALEN pair was ordered to specifically induce a DSB in the GBP1 gene, part of the GBP cluster located on chromosome 1. b. γH2AX ChIP in DIvA cells transfected either with pCDNA3 or with the GBP1-TALEN plasmids. Enrichment was measured by qPCR at 80bp from the expected DSB, or on a control genomic region. ChIP efficiency (expressed as % of input) of a representative experiment is shown. c. Levels of GBP1 mRNA measured by reverse transcription followed by qPCR, in DIvA cells treated (or not) with IFN-γ as indicated. cDNA levels were normalized to ribosomal protein P0 cDNA levels. d. H3K36me3 ChIP in control or TALEN transfected cells subjected (or not) to an IFN-γ treatment. H3K36me3 levels were analyzed by qPCR on the GBP1 gene. Mean and s.e.m (n=4, technical replicates) of a representative experiment are shown. e. XRCC4 (blue) and RAD51 (red) ChIP in GBP1-TALEN transfected cells, treated or not with γ-IFN. Enrichments of XRCC4 and RAD51 were measured by qPCR respectively at 80bp and 800bp from the TALEN-induced DSB. Mean and s.e.m (n=4, technical replicates) of a representative experiment are shown.