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. 2015 Jan;108:40–47. doi: 10.1016/j.biochi.2014.10.015

Fig. 2.

Fig. 2

Ion binding by SmTAL proteins. (a) Native gel electrophoresis of SmTAL1 (17 μM), SmTAL2 (11 μM) and SmTAL3 (32 μM). U, untreated protein; E, protein plus 1 mM EGTA; various ions as indicated at a concentration of 2 mM ion/1 mM EGTA. For the conditions of electrophoresis see Materials and Methods. Given that the proteins were shown to be dimeric in crosslinking experiments, it is reasonable to assume that the bands in these native gels also represent dimers. (b) Intrinsic fluorescence spectra of SmTAL1 (7 μM), SmTAL2 (8 μM) and SmTAL3 (7 μM) in the 0.4 mM EGTA (dashed line) and 0.4 mM EGTA/0.8 mM calcium chloride (solid line). (c) First derivative curves for the thermal denaturation (“melting”) of SmTAL1 (5 μM) and SmTAL3 (7 μM). Dashed line, protein plus 0.4 mM EGTA; solid line, protein plus 0.4 mM EGTA/0.8 mM calcium chloride.