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. 2014 Sep 5;32(2):516–527. doi: 10.1007/s11095-014-1481-5

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© The Author(s) 2014

Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Fig. 5 — Confocal micrographs with XZ stack showing distribution of junctional complex protein ZO-1 (left) and F-actin morphology (right) distribution in polarized Calu-3 monolayers following application of Solutol® HS15. White arrows highlight changes in F-actin distribution following Solutol® HS15 application. ZO-1 (green) was immunostained with mouse, anti-human ZO-1 (primary) antibody then with FITC-labelled goat, anti-mouse (secondary) antibody. F-actin was immunostained with Alexafluor 546-labelled phalloidin (red) and nuclei blue. Scale bar represents 25 μm and ‘cross hair’ represents region for Z stack analysis. ImageJ (1.47 K) image software was used to create the overlay images from raw series images.