Table 1. Activation of insulin/IGF1 receptor hybrids. The activation of insulin/IGF1 receptor hybrids were measured in HepG2 cells or in BHK cells overexpressing IGF1R and IR-A (Hybrid-A) or IR-B (Hybrid-B). The EC50 and maximal response values were calculated. The average and standard deviation were calculated with n = 4 (see Methods).

	EC50 nM		Max. response relative to IGF1 (%)
Cell line	IGF1	Insulin	IGF1	Insulin
Hybrid-A, BHK	6 ± 3	342 ± 121	100	93 ± 5
Hybrid-B, BHK	12 ± 2	325 ± 88	100	88 ± 12
HepG2	1.9 ± 1.0	42.4 ± 12.3	100	67 ± 18

BHK, baby hamster kidney; IGF1, Insulin-like Growth Factor 1.