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. 2015 Jan 21;5:7913. doi: 10.1038/srep07913

Figure 1. Promoter activity and mRNA expression of Gpr39 in the skin.

Figure 1

(a) Microscopic observation of LacZ staining in tail skin of Gpr39+/lacZ (top) and Gpr39+/+ (bottom) mice. Arrows indicate lacZ signals at the openings of SGs. (b) Whole-mount LacZ staining of an isolated intact hair follicle (HF) (left) and an HF with the sebocytes removed (right). The tissues were counter-stained with ORO. (c) RT-PCR examination of Gpr39 and Actb (as an internal control) expression in separate epidermal compartments. NEG, negative control; POS, positive control. (d) RNA in situ hybridisation of Gpr39 in the SG. (e) LacZ staining in the dorsal skin of Gpr39+/lacZ mice on different postnatal days. HF, hair follicle; SG, sebaceous gland; Epi, epidermis; HS, hair shaft; P20, postnatal Day 20. Bar (a) = 100 μm, (b) = 20 μm, (d) = 25 μm, (e) = 20 μm.