Reagent	Volume per Sample	Final Concentration
10× PCR buffer (Sigma # P2317) without MgCl2	5 Φl	1×
MgCl2 (25 mM)	3 Φl	1.5 mM
dNTP mix (10 mM each nucleotide)	1 Φl	0.2 mM each NTP
PG17.19 (5’ GAA CCA CAG CTT GAA GAC CCC GCT GA 3’)*	4 Φl	80 nM
PG17.20 (5’GCA TCT GCT GGT ACT GCT GGT AGT TG 3’)*	4 Φl	80 nM
JumpStart Taq DNA polymerase (Sigma # D4184) 2.5 unites/ul	1Φll	2.5 units
DEPC treated water	30 Φl	X

^*

from (Inui et al.,2003).