| Reagents | Volume per Sample |
Final Concentration |
|---|---|---|
| TaqMan® Universal PCR Master Mix | 12.5 | 1× |
| 18S rRNA reagent | 0.25 | 0.21× |
| DMY HTP Forward (5’ TTC TGC TGG AAA GAC 3’) | 1.75 | 0.7 nM |
| DMY HTP Reverse (5’ TCT CTG GCG GAC CAT GAT 3’) | 1.75 | 0.7 nM |
| DMY HTP Probe (5’ FAM-CCA GTG CTT CAA ATG CGA GCA-BHQ 3’) | 4.0 | 200 nM |
| RNase free water | 2.25 | -- |
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