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| Chemicals | Time (min) | Temperature (°C) |
|---|---|---|
| Fixation – GPHS fixative | Over night | 4°C + rotation |
| Dehydration | ||
| 70% ethanol | 40, 10 | RT + rotation |
| 85% ethanol | 40, 10 | RT + rotation |
| 95% ethanol | 40, 10 | RT + rotation |
| 100% ethanol | 40, 40, 10 | RT + rotation |
| Clearing | ||
| xylene | 30, 30, 30 | RT + rotation |
| Infiltration | ||
| Paraplast Plus | 60 | 58 – 60 |
| Paraplast Plus | 60 | 58 – 60 |
| Paraplast Plus | 60 | 58 – 60 |
| Paraplast Plus (embedding mold) | 60 | 58 – 60 |
Note: RT = room temperature
