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E. coli growth conditions and antibiotic study preparation and analysis workflow. Sample (A) refers to the strain stock culture, (B) NaOH control culture, (C) low dose antibiotic culture + NaOH and (D) high dose antibiotic culture + NaOH. Cultures (B), (C) and (D) had samples pulled at two time intervals – 30 minutes and 60 minutes following splitting from (A).
