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. 2014 Dec 17;15:438. doi: 10.1186/1471-2474-15-438

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© Lu et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Relative expression of key catabolic genes in the absence or presence of siHDAC4. Messenger RNA levels were quantified by real time RT-PCR and normalized to beta-actin. The expression levels of genes (A, B, C, D and E) were defined from the threshold cycle (Ct) and relative values were calculated by the 2^-ΔΔCt method. Western blot analyses (F) were used to compare the relative levels of HDAC4 protein with and without the treatment of siRNA ( C = control, NC = negative control). Data are presented as mean ± SEM(n = 3, in duplicate). **P < 0.01 ,***P < 0.001.