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Results from an dot-blot assay employing purified ITPR1 and ARHGAP26 as test substrates. IgG from the patients’ serum bound to full-length ITPR1 but not to human full-length ARHGAP26; contrarywise, anti-Ca-positive control sera bound to ARHGAP26 but not to ITPR1 (upper panel). The minimum amount of ITPR1 that resulted in a significant staining intensity was 0.45 μg/μL (middle panel). Healthy control sera bound neither to ITPR1 nor to ARHGAP26 (middle and lower panel).
