FIG 1.

Expression of miR-21 and Cdc25a in NPCs and U-251MG cells during HCMV infection. NPC (A) or U-251MG (B) cultures were infected with HCMV at MOIs of 3 or 5, respectively. Mock-infected (M) or virus-infected (V) cells were harvested at the indicated times, and the miR-21 and Cdc25a mRNA levels were quantified using qRT-PCR. The data were normalized to levels measured in mock-infected cultures to provide relative levels (V/M). The results shown are means ± 1 SD of data from three independent experiments, each conducted in triplicate. Replicate NPC (C) or U-251MG (D) cultures were evaluated by Western blotting for Cdc25a protein expression. Actin serves as a loading control.