TABLE 1.
Primers used for plasmid construction
| Plasmid | Primer sequence (5′-3′) |
|
|---|---|---|
| Forward | Reverse | |
| pCDH-miR-21-GFP | TCTAGACCTTTAGGAGCATTATGAGCAT | GGATCCAGACTATCCCCATTTCTCCAT |
| pCDH-Cdc25a-GFP | GCTAGCAGTCGTGTTTGTGTTTGACCC | GGATCCTCAGAGCTTCTTCAGACGACTG |
| pCDH-Cdc25a+WT-3′UTR-GFP | GCTAGCAGTCGTGTTTGTGTTTGACCC | GGATCCCTGCAACATTCCAGCACTGAG |
| pCDH-Cdc25a+MT-3′UTR-GFP | GCTAGCAGTCGTGTTTGTGTTTGACCC | GGATCCCTGCAACATTCCAGCACTGAG |
| pCDH-IE1-GFP | GGATCCATGGAGTCCTCTGCCAAGA | GGATCCTTACTGGTCAGCCTTGCTTCT |
| pCDH-IE2-GFP | GGATCCATGGAGTCCTCTGCCAAGA | GGATCCCTGAGACTTGTTCCT CAG |
| pCDH-pp65-GFP | GAATCCATGGAGTCGCGCGGTCGCCGTT | GAATTCTCAACCTCGGTGCTTTTTGGGCGTC |
| pCDH-pp71-GFP | TCTAGAATGTCTCAGGCATCGTCCTCG | GGATCCCTAGATGCGGGGTCGACTGCG |
| pCDH-flag-UL26-GFP | ATGGACTACAAGGACGACGATGATAAGATGACGAGCAGGCGCG | GGATCCTTACGGCAACAGCGCTGATGGC |
| pGL3-miPPR21 | GGTACCTGTATTCTGGGTAAGAAGGAGCT | CTCGAGCTTACCACCTGGACTCAAAAGG |
| pGL3cM-Cdc25a-3′UTR | GGTACCATACTGCCATTCTAGGTAGGGT | GAGCTCCTGCAACATTCCAGCACTGAG |
| pGL3cM-CCNE2-3′UTR | GGTACCAAGATTGGGTAGAACTGGTATCACT | CTCGAGCTATACAGAAGACTTCATACCGTAA |
| pLKO.1-shRNA-21-1 | CCGGAAACATTACCCAGCATCATTGCTCGAGCAATGATGCTGGGTAATGTTTTTTTTG | AATTCAAAAAAAACATTACCCAGCATCATTGCTCGAGCAATGATGCTGGGTAATGTTT |
| pLKO.1-shRNA-21-2 | CCGGATCCATATCCAATGTTCTCATTCTCGAGAATGAGAACATTGGATATGGATTTTTTG | AATTCAAAAAATCCATATCCAATGTTCTCATTCTCGAGAATGAGAACATTGGATATGGAT |
| pLKO.1-shRNA-21-3 | CCGGGACTGATGTTGACTGTTGAATCTCGAGATTCAACAGTCAACATCAGTCTTTTTG | AATTCAAAAAGACTGATGTTGACTGTTGAATCTCGAGATTCAACAGTCAACATCAGTC |
| pLKO.1-scramble | CCGGCCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTTAGGTTTTTG | AATTCAAAAACCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTTAGG |
| pcDNA3.0-UL83 | GAATCCATGGAGTCGCGCGGTCGCCGTT | GAATTCTCAACCTCGGTGCTTTTTGGGCGTC |
| pcDNA3.0-GAPDH | CGCGGATCCATGGGGAAGGTGAAGGTCGGA | CCGCTCGAGTACTCCTTGGAGGCCATGTG |