TABLE 2.
Plasmids and their applications
| Plasmid | Applications |
|---|---|
| pCDH-miR-21-GFP | Ectopic expression of miR-21 |
| pCDH-miR-UL22A-GFP | Ectopic expression of miR-UL22A |
| pCDH-Cdc25a-GFP | Ectopic expression of Cdc25a |
| pCDH-Cdc25a+WT-3′UTR-GFP | Ectopic expression of Cdc25a; impact of miR-21 on Cdc25a expression |
| pCDH-Cdc25a+MT-3′UTR-GFP | Impact of miR-21 on Cdc25a expression |
| pCDH-IE1-GFP | Ectopic expression of IE1; impact of IE1 on miR-21 |
| pCDH-IE2-GFP | Ectopic expression of IE2; impact of IE2 on miR-21 |
| pCDH-pp65-GFP | Ectopic expression of pp65; impact of pp65 on miR-21 |
| pCDH-pp71-GFP | Ectopic expression of pp71; impact of pp71 on miR-21 |
| pCDH-flag-UL26-GFP | Ectopic expression of UL26; impact of UL26 on miR-21 |
| pGL3-miPPR-21 | Impact of HCMV proteins on luciferase expression from the miR-21 promoter |
| pGL3cM-Cdc25a-3′UTR | Impact of miR-21 on luciferase expression with the 3′UTR from Cdc25a |
| pGL3cM-CCNE2-3′UTR | Impact of miR-21 on luciferase expression with a negative control 3′UTR |
| pLKO.1-shRNA-21 | miR-21 knockdown |
| pcDNA3.0-UL83 | Standard for viral genome quantification by qPCR |
| pcDNA3.0-GAPDH | Standard for cellular genome quantification by qPCR |