TABLE 3.
Primers for qPCR and qRT-PCR
| Primer | Orientationa | Sequence (5′-3′) |
|---|---|---|
| Specific RT primers | ||
| miR-21 stem-loop | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCAACAGTC | |
| U6 | GAATTTGCGTGTCATCCTTG | |
| Real-time primers | ||
| Mature miR-21 | F | TGCGTGTCGTGGAGTC |
| R | CGGGTAGCTTATCAGACTGA | |
| U6 | F | GCTTCGGCAGCACATATACTAAAAT |
| R | CGCTTCACGAATTTGCGTGTCAT | |
| Cdc25a | F | AGAGTCAACTAATCCAGAGAAGG |
| R | AGCAACTGTATGAAAGAGATAACC | |
| GAPDH | F | GAGTCAACGGATTTGGTCGT |
| R | GACAAGCTTCCCGTTCTCAG | |
| UL83 (CN) | F | GCGAGACCGTGGAACTGC |
| R | TTGCCCTGGATGCGATACTG | |
| GAPDH-(CN) | F | GACCCCTTCATTGACCTCAACTA |
| R | TCCTGGAAGATGGTGATGGG |
a
F, forward; R, reverse.