FIG 1.

WSSV PK1 interacted with host ferritin in the yeast two-hybrid system. (A) Yeast mating. The indicated pairs of plasmid constructs were transformed into two strains of yeast (Y187 and AH109) and grown on SD/−Leu/−Trp (a) and then on agar plates with higher-stringency media, i.e., SD/−Leu/−Trp/−His (b) and SD/−Leu/−Trp/−His/−Ade/+X-α-Gal (c). Growth of yeast on the two high-stringency plates was observed only with Lvferritin-pGBKT7-1/pk1-pGADT7-Rec, pk1-pGBKT7-1/Lvferritin-pGADT7-Rec, and the positive control. (B) To confirm these interactions in a cotransformation domain-swapping experiment, the indicated plasmid constructs were cotransformed into S. cerevisiae strain AH109 and screened with the highest-stringency medium, i.e., SD/−Leu/−Trp/−His/−Ade/+X-α-Gal agar. Growth of cotransformants on this medium was observed only when the yeast was cotransformed with pk1-n-pGBKT7-1/ferritin-pGADT7-Rec or with the positive control.