FIG 5.

Overexpression of PK1 in Sf9 cells elevates cellular LIP level. (A) When PK1-V5 was overexpressed in Sf9 cells, significant elevations of the LIP level occurred 38.5 h and 56.5 h after transfection. (B) Western blots with anti-V5 antibody were used to detect the PK1-V5 protein expressed in the pk1-pDHsp70/V5-His-transfected cells. The change in fluorescence (ΔF) represents the LIP level. Each bar represents the mean ± SD for 4 samples, and unpaired Student's t tests were used to test for statistically significant differences (*, P < 0.05). M, protein marker.