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Peptides cross the human airway epithelium (HAE). (A) Drops containing peptides settle at the apical side (air interface) of the HAE. Medium (liquid interface) was collected at each time point for quantification of peptide by ELISA. PVDF, polyvinylidene difluoride. (B to D) Kinetic analysis (ELISA) of the peptide concentration in the liquid reservoir of the culture, reflecting minimal transit through the HAE (MV HRC2 in panel B, MV HRC4 in panel C, and VIKIPEG4-chol in panel D).
