FIG 3.

MeV H/F₀ assembly is based on interaction of F with the H stalk domain. (A) Cell-to-cell fusion of cells transfected with MeV F and the newly generated GCN domain-stabilized H stem mutants of progressively shorter length. For comparison, the previously reported bioactive H-stem_122-GCN was included. In the case of cells expressing H-stem_110-GCN and F, arrowheads highlight individual syncytia. (B and C) Analysis of cell lysates and coprecipitated F material as outlined in the legend of Fig. 2A and B after pulldown with the specified H mutants in the presence or absence of DTSSP.