FIG 5.

DTSSP cross-linking stabilizes surface-exposed MeV glycoprotein complexes in prefusion conformation. (A) Cell-to-cell fusion of cells transfected with MeV H and F mutant F(L325D) or F(L457A-V459A). (B) F surface immunoprecipitation (SF IP) after incubation of intact cells transfected with MeV H and F(L325D) or F(L457A-V459A) with conformation-dependent antibodies directed against the F trimer in the prefusion (αPre F) or postfusion (αTrig F) conformation. (C) Analysis of cell lysates and coprecipitated F mutants F(L325D) and F(L457A-V459A) after pulldown with the specified H mutants in the presence or absence of DTSSP as outlined in the legend of Fig. 2A and B.