FIG 2.

Human SCR2 is essential for virus attachment. (A) Binding of radiolabeled CVB3-RD to CHO cells expressing human DAF (HDAF), murine DAF (mDAF), or murine DAF in which SCRs 1 to 4 have been replaced by human sequences (mouse DAF-human SCRs 1 to 4 [mDAF-HCSRs1-4]). (B) Virus binding to murine-human SCR chimeras. Chimeras are labeled according to the configuration of the human (H) and murine (m) SCR domains: for example, chimera mHHH includes murine SCR1 and human SCRs 2 to 4. Chimeras marked by an asterisk were constructed in a mouse DAF cDNA background and include the mouse DAF ST-rich domain. (C) Virus binding to human DAF from which SCR 2 or 4 was deleted (H-delSCR2 and H-delSCR4, respectively).