TABLE 3.

Number of NCCR sequences per sample retrieved by 454 sequencing

Patient no.	No. of reads	JCPyV load (no. of copies/ml)	Input copy no.a	Sampling sizeb
1	2,715	69,718	50,196	5.41
1	3,074	5,562,426	2,669,964	0.12
1	3,337	179,375,000	43,050,000	0.01
1	3,500	172,000,000	41,280,000	0.01
1	2,588	>225,000,000	>54,000,000	>0.01
2	2,512	278,000,000	33,360,000	0.01
2	1,511	51,006	14,690	10.29
2	1,390	26,912	7,750	17.94
3	6,195	96,438	46,290	13.38
3	1,932	10,812	10,380	18.61
3	1,860	4,063	3,900	47.69
4	160	5,035	1,450	11.03
4	622	2,238,800	1,074,624	0.06
5	1,677	558,967	536,608	0.31
5	1,567	756,637	453,982	0.35
5	1,453	12,728	12,219	11.89
6	3,265	29,584	14,200	22.99
7	4,839	15,912	15,276	31.68
8	3,515	100,046	96,044	3.66
9	3,982	1,627,025	488,108	0.82
10	2,163	26,633	25,568	8.46
11	1,810	181,566	174,303	1.04
12	809	656,020	472,334	0.17
13	2,987	2,501,045	1,500,627	0.20
14	5,931	72,734	34,912	16.99
15	4,771	43,694,938	10,486,785	0.05
16	5,196	94,545,825	22,690,998	0.02
17	5,834	189,945,812	45,586,995	0.01
18	5,583	7,744,225	1,858,614	0.30
19	5,391	2,804,051	1,345,944	0.40

^aThe number of viral copies used for triplicate amplification of the JCPyV NCCR.

^bSampling size is defined as [(number of reads/input copy number) × 100].