FIG 1.

p012 is generated from a spliced transcript. Shown are the position of MDV ORF012 and the splicing of its mRNA. The structure of the MDV genome is outlined, and the positions of the hypothetical ORF011* and ORF012* genes (black) in relation to other genes are indicated. Sizes are given in base pairs (bp) for DNA or bases (b) for RNA. The 82-bp intron in the former ORF011* is indicated in gray. The predicted splicing results in a frameshift and absence of the predicted ORF011* stop codon. Splicing results in fusion of the remaining sequence with the formerly predicted short intergenic region and the 5′ end of the former ORF012*, thus creating the novel ORF012 transcript. The splice donor (GT) and acceptor (AG) sites are indicated in the intron. The primary sequence of p012 with predicted phosphorylation sites (underlined) and a predicted NLS (boxed) is shown. Initiator methionines of p012 and the former p012*, respectively, are marked in boldface. The amino acid corresponding to the exon-exon border (arginine; R) is marked in boldface and italics.