FIG 8.
Leptomycin B inhibits nuclear export of p012. (A) DF-1 cells were transfected with pc012Flag. At the indicated time points, cells were fixed and stained. The results of two independent experiments are shown. (B) DF-1 cells were transfected with pc012Flag. At 6 h or 10 h after transfection, the cells were incubated with 2 μM or 20 μM LMB and treated for 9 h or 5 h, respectively, before fixation and staining. The combined results of 5 independent experiments are shown as percentages of cells with predominantly cytoplasmic localization. Lines and error bars represent the mean values and the standard error of the mean (SEM), respectively. Note that the axis is scaled to 50%. Differences were tested for significance by χ2 tests (***, P < 0.01).