TABLE 1.
Primers used in this study
| Primer | Sequence (5′→3′)a |
|---|---|
| TS1 | ATGACTAGCGAGAGAGCTCTTACTCT |
| TS2 | TGTACGCCAAATTTTACAACGATTAT |
| TS3 | CTATTCATCATCTGAACTCGACATCC |
| chGAPDH for | ATGGTGAAAGTCGGAGTCAACG |
| chGAPDH rev | TCACTCCTTGGATGCCATGTG |
| vRΔ012 for | AACGAGAGGTTGGTAACAAACAGCTTTTGAAAATAAACTAGCGAGAGAGCTAGGGATAACAGGGTAATCGATTT |
| vRΔ012 rev | TACCAGGCGCGAGAGTAAGAGCTCTCTCGCTAGTTTATTTTCAAAAGCTGGCCAGTGTTACAACCAATTAACC |
| vRΔ012R for | AACGAGAGGTTGGTAACAAACAGCTTTTGAAAAATGACTAGCGAGAGAGCTAGGGATAACAGGGTAATCGATTT |
| vRΔ012R rev | TACCAGGCGCGAGAGTAAGAGCTCTCTCGCTAGTCATTTTTCAAAAGCTGGCCAGTGTTACAACCAATTAACC |
| v20_012Flag for | AGATCTTGTGGTTCTTGGGATGTCGAGTTCAGATGATGAAGACTACAAAGACGATGACGACAAGTAGCATTTGCCAGTGTTACAACCAATTAACC |
| v20_012Flag rev | ACAGTGGATTTGCAATCACACAACATATACACAAATGCTACTTGTCGTCATCGTCTTTGTAGTCTTCATCATTAGGGATAACAGGGTAATCGATTT |
| v20_012ΔNLSFlag for | CTTGGATACCGTTGTCGTTCGAGATCACCCAGTAACACATGACTACAAAGACGATGACGAGCCAGTGTTACAACCAATTAACC |
| v20_012ΔNLSFlag rev | ATATACACAAATGCTACTTGTCGTCATCGTCTTTGTAGTCATGTGTTACTGGGTGATCTCTAGGGATAACAGGGTAATCGATTT |
| v20_012mutshortNLSFlag for | ATAACAGTGAAGATCCAAACCGTAGTCGGAGCCGGAGTCGATCTAGGGAGGCAGCGGCAGCAGCCGCAGCAGTTAGGCCTGCCAGTGTTACAACCAATTAACC |
| V20_012mutshortNLSFlag rev | CCACAAGATCTCGTATAGTTGTAGCCGTACTCCTACGCCCAGGCCTAACTGCTGCGGCTGCTGCCGCTGCCTCCCTAGATTAGGGATAACAGGGTAATCGATTT |
| 012*Flag for | ACTCGAGCGGCCGCGCCACCATGTTTACCGGAGGAGGAACTATTG |
| 012*Flag rev | GAGCTCGGATCCTTACTTGTCGTCATCGTCTTTGTAGTCTTCATCATCTGAACTCGACATCCC |
| 012ΔintFlag for | CTCGAGCGGCCGCGCCACCACCATGACTAGCGAGAGAGCTCTTACTCTCGCGCCTGGTAAAGTTTCGACGGCAGATATTTATGAAGCCGATTTCAGTTTCCGTCGTGAATTTGTACGCCAAATTTTACAACGATTATTCCCAAGGACCTT |
| 012ΔintFlag rev | AACTTAAGCTTCTACTTGTCGTCATCGTCTTTGTAGTCTTCATCATCTGAACTCGACATCCCA |
| GFPcterm for | CAGATCTCGAGTAGTTCGAGATCACCCAGTAACACATCG |
| GFPcterm rev | AATTCGAAGCTTTTATTCATCATCTGAACTCGACATCCC |
| GFP_GSlinker for | CAGATCTCGAGCTCAAGGAGGCAGTGGTGGAGG |
| GFPlongNLS template | AAGGAGGCAGTGGTGGAGGCAGTGGTCGTAGTCGGAGCCGGAGTCGATCTAGGGAGCGTAGGCGAAGACGGCCACGAGTTAGGCCTGGGCGTAGGTAA |
| GFPlongNLS rev | TCGACTGCAGAATTCTTACCTACGCCCAGGCCTAAC |
| GFPshortNLS template | AAGGAGGCAGTGGTGGAGGCAGTGGTCGTAGGCGAAGACGGCCACGATAA |
| GFPshortNLS rev | GTCGACTGCAGAATTCTTATCGTGGCCGTCTTCGC |
| GFP_RSrepeat template | GAGCTCAAGGAGGCAGTGGTGGAGGCAGTGGTCGTAGTCGGAGCCGGAGTCGATCTAGGGAGTAAGAATTC |
| GFP_RSrepeat rev | TCGACTGCAGAATTCTTACTCCCTAGATCGACTCCGG |
| 012mutshortNLS for | TCGGAGCCGGAGTCGATCTAGGGAGGCTGCGGCAGCAGCGGCAGCAGTTAGGCCTGGGCGTAGGAGTACG |
| 012mutshortNLS rev | CGTACTCCTACGCCCAGGCCTAACTGCTGCCGCTGCTGCCGCAGCCTCCCTAGATCGACTCCGGCTCCGA |
| 012mutRSrepeat for | ACAGTGAAGATCCAAACGCCGCAGCGGCTGCAGCAGCTGCCAGGGAGCGTAGGCGAAGACGG |
| 012mutRSrepeat rev | CCGTCTTCGCCTACGCTCCCTGGCAGCTGCTGCAGCCGCTGCGGCGTTTGGATCTTCACTGT |
| 012mutStoA for | GTGAAGATCCAAACCGTGCTCGGGCCCGGGCTCGAGCTAGGGAGCGTAGGCGA |
| 012mutStoA rev | TCGCCTACGCTCCCTAGCTCGAGCCCGGGCCCGAGCACGGTTTGGATCTTCAC |
a
Regions of interest (restriction sites, mutated sequences, epitope tags, and sequences representing the exon-exon border of ORF012 [primer TS2]) are underlined.