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Intracellular localization of gH. RK13 cells were transfected with gH expression plasmids and analyzed by indirect immunofluorescence. Two days after transfection, the cells were fixed with 3% paraformaldehyde (upper panels) and additionally permeabilized with 0.2% Triton X-100 (lower panels). Surface-localized and intracellular gH was detected using a monospecific rabbit antiserum and Alexa Fluor 488-conjugated secondary antibodies.
