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Western blot analyses of purified PrV particles. Virion proteins of pPrV-gHK (WT) and the indicated gH mutants were separated by SDS-PAGE. Blots were incubated with gH-, gB- or gL-specific rabbit antisera. The signals of mature gH, full-length gB (gBa), and furin-cleaved subunits (gBb, gBc) as well as gL are labeled by arrows, and molecular masses of marker proteins are indicated.
