TABLE 1.

Oligonucleotide primers^a

Primer use and name	DNA sequence, 5′ → 3′	Nucleotide positions
Site-directed mutagenesisb
PGHV174C-F	CTCGGCCTGCGCTGCGCGCTGCGCTC	61,117–61,142
PGHA270C-F	CTACTTTTACCGCGCATGCGTGCGCCTCGGCGTGGC	61,401–61,436
PGHA252C-F	CGGCGGCGCACTGTGCCGCCCTGGCC	61,352–61,377
PGHA419C-F	GAGCTCACGCGGGCATGCCTCTCGCCGACGG	61,849–61,879
PGHH251C-F	GCTGTCGGCGGCATGCGCGGCCGCCC	61,347–61,372
PGHH251S-F	GCTGTCGGCGGCTAGCGCGGCCGCCC	61,347–61,372
PGHL432C-F	CGAGCCCTTCAGCTGCCTCGACGTCCTCTCG	61,890–61920
PGHA250C-F	GCCCAGCTGTCGGCATGCCACGCGGCCGCC	61,342–61,371
PGHA250S-F	CCAGCTGTCGGCTAGCCACGCGGCCGC	61,344–61,370
PGHA276C-F	CGCCTCGGCGTGTGCGCATTCGTCTTCTCCG	61,423–61,453
PGHA253C-F	CGGCGCACGCGTGCGCCCTGGCCGC	61,355–61,379
PGHA269C-F	CGTACTACTTTTACCGCTGCGCAGTGCGCCTCGGCG	61,397–61,432
PGHA284C-F	CGTCTTCTCCGAGGCATGCCGCCGCGACCGGC	61,443–61,474
PGHA284S-F	CTTCTCCGAGGCTAGCCGCCGCGACCG	61,446–61,472
PGHS291C-F	GACCGGCGCGCCTGTGCACCGGCGCTCC	61,468–61,495
PGHA250P-F	CAGCTGTCGGCGCCGCACGCGGCC	61,345–61,368
PGHV298P-F	CGGCGCCGGCGCTCCTGAGGCCTGAGAGCGACGCGCGCC	61,481–61,519
PGHV275P-F	GTGCGCCTCGGCCCGGCCGCCTTCG	61,420–61,444
PGHK-DRDF	GGGAGACCCAAGCGTCTTCAAAGTTTGCCGTGCCC	(pcDNA3)
PCR amplification and sequencing
PGH-PSF	TTCACGTCGGAGATGGGG	60,451–60,468
PGH-PSF2	GGAAGCCCTTCGACCAG	61,715–61,731
PGH-PSR	AGTTATGTCATCCAGCAGCC	62,887–62,868 (r)
PGH-PSR2	GTCGAGCAGGCTGAAGG	61,911–61,895 (r)
PGH-WH3	TGCACGAGAGCGACGACTACC	61,319–61,339
PGHK-PSF	TGACGAGCGTAATGGCTGG	(pACYC177)
PGHK-PSR	CGCGAGCCCATTTATACCC	(pACYC177)
KanR-F	TCCGGATCCCGATTTATTCAACAAAGCCACG	(pACYC177)
KanR-R	TTCGAATTCGCCAGTGTTACAACCAATTAACC	(pACYC177)
T7	TAATACGACTCACTATAGGG	(pcDNA3)
SP6	CTCTAGCATTTAGGTGACACTATAG	(pcDNA3)

^aArtificial restriction sites used for cloning or fragment isolation are underlined. Altered nucleotides are printed in bold letters. Nucleotide positions refer to GenBank accession number BK001744.

^bForward primer sequences are shown for site-directed mutagenesis. The corresponding reverse primers were exactly complementary.