TABLE 1.
Intrathoracic infection of C. quinquefasciatus with SINV-eGFP and SINV/EILV chimerasa
| Virus | Dose (log10 PFU/μl) | % infected (no. of infected mosquitoes/total no. of mosquitoes) (7 dpi) |
|---|---|---|
| SINV-eGFP | 3.9 | 100 (12/12) |
| 3.5 | 100 (15/15) | |
| 2.2 | 86 (12/14) | |
| 1.4 | 77 (10/13) | |
| SINV-eGFP with EILV 3′ UTR | 3.8 | 100 (15/15) |
| 3.4 | 100 (15/15) | |
| 2.4 | 87 (13/15) | |
| 1.4 | 80 (12/15) | |
| SINV-eGFP with EILV structural ORF | 4.0 | 100 (15/15) |
| 3.6 | 100 (15/15) | |
| 2.5 | 80 (12/15) | |
| 1.4 | 73 (11/15) |
a
C. quinquefasciatus mosquitoes were injected with ∼104 to 101 PFU/mosquito via the intrathoracic route, and at 7 dpi, the presence of infectious virus was detected by cytopathology and eGFP expression.