FIG 3.

(A) Knockdown of HPV-18 E6 in HeLa cells increases nuclear PDZRN3 levels. HeLa cells were seeded onto coverslips and transfected with siRNA for luciferase or HPV-18 E6/E7 (Dharmacon). After 48 h, the cells were fixed, permeabilized, and then probed for p53 (red) and PDZRN3 (green). Each panel shows two different fields of view. (B) In C33A cells, transfection of HPV-18 E6 reduces nuclear PDZRN3 in a PBM-dependent manner. C33A cells were seeded onto coverslips and transfected with pCDNA3.1, pCDNA3:HA-HPV-18E6, or pCDNA3:HA-HPV18E6T156E. After 24 h, the cells were fixed, permeabilized, and then probed for HA (red) and PDZRN3 (green). Arrows indicate cells expressing E6 with a corresponding reduction in nuclear PDZRN3 with wild-type E6 but no change with the T156E mutant.