FIG 2.

(A) Summary of the neutralization sensitivities of parental and corresponding A204E/G312V mutant Env proteins to MAbs 17b, VRC03, 697-30D, and 447-52D and to HIV⁺ pooled plasma. The envelope clone and the virus subtype (in parentheses) are indicated in the first column. The A204E and G312V mutant proteins are indicated below the corresponding parental Env protein. Darker shading indicates increasing sensitivity to the MAb tested or increasing sensitivity to HIV⁺ pooled plasma, according to the key at the bottom. Gray shading indicates that 50% neutralization was not reach at the highest concentration tested (10 μg/ml) for MAbs or the highest dilution of plasma tested (1:100). Comparison of IC₅₀s for parental and A204E mutant Env proteins for MAbs 17b (B), 697-30D (C), 447-52D (D), and VRC03 (E). IC₅₀s (μg/ml) are plotted for the parental (left) and corresponding A204E mutant (right) Env proteins. The key at the right indicates the color coding of each parental and mutant Env pair tested.