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. 2014 Nov 5;89(2):894–907. doi: 10.1128/JVI.02680-14

FIG 5.

FIG 5

Evaluation of CD4-independent Env proteins from QA255.662M.C (A) Cell-cell fusion activity of CD4-independent Env proteins was assessed on QT6 target cells expressing the indicated coreceptors with or without CD4. Fusion was quantified in relative light units (RLU). A vector containing only GFP was used as a negative control. (B) Viral growth in CD4-positive (SupT1/CCR5) and CD4-negative (BC7/CCR5) cell lines is shown for subtype A viruses containing parental QA255.662M.C or CD4-independent QA255-CD4iA and QA255-CD4iB Env proteins. Reverse transcriptase (RT) activity (counts per minute [cpm]) is shown. (C, D) Summary of neutralization profiles of CD4-independent Env proteins. The parental Env protein from which the CD4-independent Env proteins were derived is indicated on the first row with the CD4-independent Env proteins below.